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. 2015 Feb;22(2):109–115. doi: 10.1101/lm.037044.114

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© 2015 Briand et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Ser133Ala mice exhibit normal levels of CREB-binding and gene transcription. Chromatin immunoprecipitation revealed normal levels of CREB enrichment at the cfos (A) F_(2,13) = 49.01, P < 0.0001; S133WT, S133A Mutant versus Control, P < 0.0001; S133WT versus S133A Mutant, NS) and fosB (B) F_(2,13) = 22.38, P < 0.0001; S133WT, S133A Mutant versus Control, P < 0.01; S133WT versus S133A Mutant, NS) promoter regions, with no significant differences between Ser133Ala mice and controls. Normal levels of HK3K4me3 enrichment at the cfos (C) F_(2,13) = 15.32, P = 0.0009; S133WT, S133A Mutant versus Control, P < 0.01; S133WT versus S133A Mutant, NS) and fosB (D) F_(2,13) = 21.40, P = 0.0004; S133WT, S133A Mutant versus Control, P < 0.01; S133WT versus S133A Mutant, NS) promoter in Ser133Ala mutant mice indicate a transcriptionally permissive state. Fear conditioning leads to a significant increase in Nr4a1 (E) N = 5–8; F_{INTERACTION (3,42)} = 6.056, P = 0.0016, S133 WT, S133A Mutant, CREBαΔ Fear versus Control, P < 0.001; CREBαΔ versus Control, NS), Nr4a2 (F) N = 5–8; F_{INTERACTION (3,44)} = 9.566, P < 0.0001, S133 WT, S133A Mutant, CREBαΔ Fear versus Control, P < 0.0001; CREBαΔ versus Control, NS), and cfos (G) N = 5–8; F_{INTERACTION (3,44)} = 5.87, P = 0.006, S133 WT, S133A Mutant, CREBαΔ Fear versus Control, P < 0.0001; CREBαΔ versus Control, NS) hippocampal gene expression in both wild-type and Ser133Ala mice but not CREBαΔ mutant mice. (**) P < 0.01, (***) P < 0.001 compared with tissue taken from naïve controls. Global expression profiling of hippocampal lysates from S133A versus wild-type mice revealed no significant alterations in any individual gene on the microarray (H).