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U2OS cells stably expressing G3BP-GFP (SG marker) and Dcp1-RFP (P-body marker) were treated with 200 nM Tg for 40 min and then 200 nM ISRIB was added at t = 0 min to the well and imaged using an epifluorescence microscope. Images of G3BP-GFP (SGs) were collected every 30 s.
