Figure 7.

iCRT14 rescues aberrant AURKA signaling in RCC cell lines. (A) RT-PCR analyses showing AURKA, CyclinD1, c-myc, and HDAC6 mRNA expression in VHL-null, 786-O, and 769-P RCC cell lines treated with vehicle (EtOH; black bars) or iCRT14 (15 μM; gray bars) (P<0.05). (B) Immunoblot analyses from 786-O and 769-P RCC cells treated with EtOH (black bars) or iCRT14 (gray bars), probed with the indicated antibodies (left). The graph (right) represents densitometric analyses from at least four independent replicates showing levels of protein expression normalized to GAPDH expression (loading control) (P<0.05). (C) Model for β-catenin regulation of AURKA in VHL-deficient cells. *Statistically significant differences.