Figure 1.
EPO gene expression in isolated glomeruli and EPO in situ hybridization on kidney sections of Ren1+/Cre and Ren1+/Cre Vhlfl/fl mice. EPO mRNA abundance of isolated glomeruli is given as ratio over RPL32 mRNA (upper panel), which is considered as a standard. The ratio of control values is set to 1. Data are the mean±SEM of six mice in each group. *P<0.05 by the t test. Localization of EPO mRNA expression by performing in situ hybridization on kidney sections of Ren1+/Cre and Ren1+/Cre Vhlfl/fl mice (lower panel). Arrows indicate JG cells/vascular poles. Arrowheads show JG EPO-expressing cells. aa, afferent arteriole; G, glomeruli; ISH, in situ hybridization; RPL32, ribosomal protein L32. Scale bar, 50 µm.