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. 2014 Jul 28;26(3):587–596. doi: 10.1681/ASN.2013111152

Figure 6.

Figure 6.

The endocrine switch of juxtaglomerular cells in the Ren1+/Cre Vhlfl/fl mice depends on HIF-2α accumulation. Abundance of HIF-2α protein in isolated glomeruli (A), plasma renin concentration (B), plasma EPO concentration (C), and hematocrit values (D) of Ren1+/Cre, Ren1+/Cre Vhlfl/fl, Ren1+/Cre Vhlfl/fl HIF-2αfl/fl, and Ren1+/Cre HIF-2αfl/fl mice. Renin cell-specific Vhl knockout mice have higher glomerular HIF-2α levels, increased plasma EPO and hematocrit values, but lowered plasma renin concentrations compared with the Ren1+/Cre control mice. These changes in the Ren1+/Cre Vhlfl/fl mice are almost completely reversed by an additional knockout of HIF-2α. Knockout of HIF-2α alone in the renin cell lineage has no influence on renin or EPO expression. Hematocrit values are in line with the EPO data. Data are the mean±SEM of six mice in each group. For determination of HIF-2α abundance, glomerulus preparations from two animals are pooled (see the Concise Methods). After cell lysis and immunoblotting, membranes are incubated with antibodies against HIF-2α or β-actin as a standard (see Supplemental Figure 1). Band intensities are quantified measuring OD on a Gel Doc 2000 (Bio-Rad) and analyzed by Multi-Analyst Software (Bio-Rad). The ratio of control values (glomerulus preparations of Ren1+/Cre mice) is set to 1. *P<0.05.