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. 2014 Jul 28;26(3):647–662. doi: 10.1681/ASN.2013111195

Figure 1.

Figure 1.

Rhophilin-1 is expressed exclusively in podocytes of the kidney glomerulus. (A) In human glomeruli, Rhophilin-1 localizes extensively with the podocyte slit diaphragm protein nephrin. (B) In mouse, Rhophilin-1 is expressed in the glomerulus, where it surrounds and extends from WT1-expressing podocytes. (C) Rhophilin-1 colocalizes with the podocyte-specific protein dendrin in foot processes of the mouse glomerulus (high magnification in lower panel). (D) RT-PCR analysis of YFP-positive (+) and YFP-negative (−) cell populations obtained from FACS-sorted glomerular cells of Nphs2-cre–driven ROSA26/YFP reporter mice. Only + cells express Nphs1 and Rhpn1, whereas only − cells express the endothelial-specific transcript Ehd3. Glomeruli (G) express each of the transcripts. DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Scale bars, 20 μm in A; 10 μm in B and C, upper panel; 2 μm in C, lower panel.