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. 2014 Aug 21;26(3):576–586. doi: 10.1681/ASN.2014040340

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Figure 3. — Verification of Vangl2 excision in developing podocytes by immunofluorescence microscopy. (Upper panel) Low-magnification images of control (Vangl2^flox/flox;Cre^–) and knockout (Vangl2^flox/flox;Pod-Cre) E17.5 kidney sections costained with anti-Vangl2 antibody (red) and antinephrin antibody (green). Yellow arrowheads point to the tubular structures positive for Vangl2 protein in both control Vangl2^flox/flox;Cre^– and knockout Vangl2^flox/flox;Pod-Cre mice. DAPI, 4',6-diamidino-2-phenylindole. (Lower panel) High-magnification images of the capillary loop stage glomerulus (designated by an intermittent white line) in control Vangl2^flox/flox;Cre^– and knockout Vangl2^flox/flox;Pod-Cre kidneys. Note the absence of a Vangl2-positive signal (white arrows) in the glomerulus of the Vangl2^flox/flox;Pod-Cre sections, confirming successful excision of the Vangl2 gene. Scale bars,10 μm in upper panel; 5 μm in lower panel.