Table 1.
Strains, phages, and plasmids used in this study.
| Strain | Genotype or relevant characteristica | Reference or source |
|---|---|---|
| Serratia plymuthica A153 strains | ||
| A153 | Wild-type, rhizosphere isolate (Zea+) | Åström and Gerhardson (1988) |
| A153Lb | lac- derivative of A153 | Matilla and Salmond (unpublished results) |
| A153A | lac-,ΔandR, made by marker exchange mutagenesis | Matilla and Salmond (unpublished results) |
| A153C | lac-,ΔcsrB | Matilla and Salmond (unpublished results) |
| A153H | lac-,Δhfq, made by marker exchange mutagenesis | Matilla and Salmond (unpublished results) |
| A153AH | lac-,ΔandRΔhfq, made by marker exchange mutagenesis | Matilla and Salmond (unpublished results) |
| A153P | lac-,ΔpigP::Km, Kmr | Matilla and Salmond (unpublished results) |
| A153R | lac-,ΔrpoS::Km, Kmr | Matilla and Salmond (unpublished results) |
| A153T6 | lac-; 1.259 bp deletion of the promoter region of the two predicted transcriptional units of the T6SS of A153; made by marker exchange mutagenesis | Matilla and Salmond (unpublished results) |
| A153Ce10 | lac-, zmn16::mini-Tn5Sm/Sp, Zea-, Smr | This study |
| A153Ce10A | lac-,ΔandR, zmn16::mini-Tn5Sm/Sp, Zea-, Smr; derivative of A153A following transduction using ΦMAM1 grown on strain A153Ce10 | This study |
| A153JH1 | lac-, ΔandR, zmn16::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH5 | lac-, ΔandR, zmn18/19::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH6 | lac-, ΔandR, zmn13::Tn-KRCPN1lacZ, Zea-; Kmr | This study |
| A153JH6H | lac-, ΔandR, Δhfq, zmn13::Tn-KRCPN1lacZ, Zea-, Kmr; derivative of A153AH following transduction using ΦMAM1 grown on strain A153JH6 | This study |
| A153JH8 | lac-, ΔandR, ydhI::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH9c | lac-, ΔandR mpg::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH10 | lac-, ΔandR, ydhJ::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH11c | lac-, ΔandR, far::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH14 | lac-, ΔandR ydhI::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH23 | lac-, ΔandR ydhI::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH24 | lac-, ΔandR zmn10::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH27 | lac-, ΔandR zmn9::Tn-KRCPN1, Zea-; Kmr | This study |
| A153JH28 | lac-, ΔandR zmn10::Tn-KRCPN1, Zea-; Kmr | This study |
| Serratia strains | ||
| S. plymuthica AS9 | Wild-type | Neupane et al. (2012a) |
| S. plymuthica AS12 | Wild-type | Neupane et al. (2012b) |
| S. plymuthica AS13 | Wild-type | Neupane et al. (2012c) |
| Escherichia coli strains | ||
| OP50 | uracil auxotroph | Brenner (1974) |
| β2163 | F- RP4-2-Tc::Mu ΔdapA::(erm-pir), Kmr | Demarre et al. (2005) |
| Bacillus subtilis strains | ||
| JH642 | pheA1 trpC2 | Perego et al. (1989) |
| Dickeya strains | ||
| Dickeya sp. MK7 | Wild-type | Pritchard et al. (2013b) |
| Dickeya solani MK10 | Wild-type | Pritchard et al. (2013a) |
| Dickeya solani MK16 | Wild-type | Pritchard et al. (2013a) |
| Dickeya solani IPO 2222 | Wild-type | Pritchard et al. (2013a) |
| Dickeya sp. NCPPB 3274 | Wild-type | Pritchard et al. (2013b) |
| Dickeya sp. CSL RW 240 | Wild-type | Pritchard et al. (2013a) |
| Fungal strains | ||
| Verticillium dahliae 5368 | Wild-type, plant pathogen | J. Cooper |
| Saccharomyces cerevisiae | Wild-type | S. Oliver |
| Schizosaccharomyces pombe | Wild-type | J. Mata |
| Caenorhabditis elegans | ||
| DH26 | fer-15-(b26) | Caenorhabditis genetics center |
| Bacteriophage | ||
| ΦMAM1 | Generalized transducing phage for S. plymuthica A153 | Matilla and Salmond (2014) |
| Plasmids | ||
| pKRCPN1 | Kmr, Tcr; Derivative of pDS1028uidA with the uidA and cat genes replaced with ´lacZ and km genes | Roberts (2010) |
| pUT-mini-Tn5-Sm/Sp | Delivery plasmid for mini-Tn5Sm/Sp, Apr, Smr | de Lorenzo et al. (1990) |
| pTRB30 | pQE-80L (Quiagen)-based expression vector, Apr replaced by Kmr, Kmr | T. Blower |
| pJEEUH13 | hfq expression vector, Kmr | This study |
aAp, ampicillin; Km, kanamycin; Sm, streptomycin; Tc, tetracycline; Zmn, zeamine. bStrain A153L is considered the A153 wild-type precursor of derivatives used in this study and is referred to as such throughout. cTransposon insertions in these strains were in previously unlabelled genes, and have been named as follows: A153JH9, mannose-1-phosphate guanyltransferase (mpg); A153JH11, ferric aerobactin receptor (far).