FIG. 2.

Single transposon insertion in CgTn201S. Rescued plasmid pTn210S and the genomic DNAs of Cg1660, CgTn201S, and complemented strain CgTn201C were digested with BglII, electrophoresed, and blotted onto a nylon membrane. The sizes of the DNA fragments detected are indicated in kilobases, and the insertion of a single transposon with its flanking regions of CgERG1 is shown. (A) Restriction enzyme map of CgERG1 locus in the wild-type strain (Cg1660) and the mutant into which the transposon was inserted (CgTn201S); (B) Southern blot analysis with the 2.9-kb transposon Tn5<Cm URA3> (hatched box in panel Biosciences) as the probe; (C) Southern blot analysis with the 2.2-kb CgERG1 sequence (black box in panel A) as the probe.