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Construction of S. cerevisiae recombinant strains with FOL1 gene with a mutation in the DHPS domain. Strain EHY1 was transformed with pRS406 linearized with BglII, which carried part of the FOL1 gene with a wild-type or mutated (M1, M2, or M3) DHPS domain. The horizontal arrows represent PCR primers used for controlling the genetic structure of the recombinant strains.
