TABLE 1.
IC50s of sulfa drugs in liquid media for recombinant S. cerevisiae strains with wild-type or mutated FOL1 genes
| Sulfa drug | IC50 (μg/ml)a for strain:
|
|||
|---|---|---|---|---|
| WT | M1 | M2 | M3 | |
| Sulfamethoxazole | 61.5 ± 24.8 | 9.1 ± 1.4 | 11.0 ± 2.9 | −b |
| Sulfamethoxazole + 0.05 μg of PABA/ml | >800c | 63.7 ± 31.8 | 268.1 ± 28.7 | 60.5 ± 23.7 |
| Dapsone | >800 | 54.8 ± 42.7 | 102.9 ± 26.8 | − |
| Sulfadoxine | 123.8 ± 53.1 | 17.2 ± 7.3 | >800 | − |
| Sulfanilamide | 286.8 ± 115.6 | >800 | >800 | − |
a
Sulfa drug resistance was tested in liquid minimal medium lacking PABA or supplemented with 0.05 μg of PABA/ml as described in the legend to Fig. 7. Each value is the average of at least two independent experiments ± standard deviation.
b
−, not measurable because there was no growth in the absence of PABA.
c
Highest concentration without precipitation of sulfa drug.