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. 2004 Jul;48(7):2581–2587. doi: 10.1128/AAC.48.7.2581-2587.2004

FIG. 2.

FIG. 2.

Genotyping of pvdhfr by melting curve analysis of 134 P. vivax-infected samples. (A) Melting temperature peaks of the alleles obtained for the F57I/L, S58R, and T61M mutations: a, wild-type alleles F57, S58, and T61; b, mutant type 1 alleles F57, S58R, and T61; c, mutant type 2 alleles F57L, S58R, and T61M; d, mutant type 3 alleles I57L, S58R, and T61M. (B) Melting peaks of the alleles for S117N/T. e, wild-type allele S117; f, mutant type 1 allele S117N; g, mutant type 2 allele S117T. (C) Melting peaks obtained for mutations I172V and I173L. h, wild-type alleles I172 and I173; i, mutant type alleles I172 and I173L. (D) Double peaks indicating the presence of both wild-type and mutant alleles simultaneously in the same sample.