TABLE 1.
Sequences of primers and oligonucleotides probes used for detection of pvdhfr mutations F57L/I, S58R, T61M, S117N/T, I172V, and I173L
| Oligonucleotide | Sequence (5′-3′)a | Position |
|---|---|---|
| dhfrS | TCTGGGCAATAAGGGGACT | 114-132 |
| dhfrA | AGTTTCTACTTAGGCATTCCCTAT | 559-536 |
| sensor 57/8 | GTAGGTCGTCACCGAGCTGAAGT-FL | 189-167 |
| anchor 57/8 | LC Red640-CTTCATATCGACGGAGTTGCATTTCCATG-PH | 165-137 |
| sensor [G] | GATGCTCTCCCAGCTGCTTC-FL | 363-344 |
| anchor 117 | LC Red640-CCCCATGACCACGACGTTTTGCAG-PH | 342-319 |
| sensor 172V | TGTGCTCCCCCAATGACGA-FL | 530-512 |
| anchor 172/3 | LC Red640-GCATTTGTAGTACTTCAGCTTCTTTAAGAGC-PH | 510-480 |
a
FL, fluorescein; LC Red640, LightCycler dye Red640; PH, phosphate; boldface, mutated nucleotides.