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. Author manuscript; available in PMC: 2015 Feb 26.
Published in final edited form as: J Immunol Methods. 2010 Oct 31;374(0):5–12. doi: 10.1016/j.jim.2010.10.012

Figure 1.

Figure 1

Schematic diagram of the dissociation assay. Biotinylated MHC-I heavy chain binds to the surface of streptavidin scintillation microplates (FlashPlates PLUS). In the presence of a binding peptide, radiolabeled β2m is bound to the MHC-I heavy and the beta-radiation can reach the scintillant embedded in the microplate resulting in a scintillation signal.