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. Author manuscript; available in PMC: 2015 Feb 26.
Published in final edited form as: Radiat Res. 2015 Jan 7;183(1):114–123. doi: 10.1667/RR13807.1

FIG. 4.

FIG. 4

Effect of nonradiosensitizing concentrations of dFdCyd on dATP levels in CHO cells. AA8 and irs1SF cells were incubated with IC10 or IC50 for dFdCyd based on either a 4 or 10 h incubation. At the conclusion of the incubation, dNTPs were extracted and analyzed by HPLC. After exposure to 4 h IC10 or IC50, dATP was measured in AA8 cells (panel A) and irs1SF cells (panel B). A time course was performed to determine the kinetics of dATP depletion in response to the 4 h IC50 dFdCyd in AA8 cells (panel C) and irs1SF cells (panel D). In addition, dATP was measured after exposure to the 10 h IC10 and IC50 in AA8 cells (panel E) and irs1SF cells (panel F). Values represent the amount of dATP in dFdCyd-treated cells expressed as a percentage of that in untreated control cells.