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. Author manuscript; available in PMC: 2016 Mar 18.
Published in final edited form as: Toxicol Lett. 2015 Feb 7;233(3):258–266. doi: 10.1016/j.toxlet.2015.02.005

Fig. 5.

Fig. 5

4-ClBQ-induced CYP1A1 mRNA expression is AhR dependent in HaCaT cells. (A) Knockdown of AhR mRNA expression using AhR-siRNA. HaCaT cells were transfected with 50 nM of control or AhR siRNA. After 48 h of transfection, cells were harvested for measurements of AhR mRNA levels by using a quantitative RT-PCR assay. Asterisks represent statistical significance compared to cells that were transfected with control siRNA; p < 0.05, n = 3. (B) AhR knockdown significantly inhibits 4-ClBQ-induced increases in CYP1A1 mRNA expression. Control and AhR siRNA transfected cells were treated with 4-ClBQ for 24 h and CYP1A1 mRNA levels were measured using a quantitative RT-PCR assay. Asterisks represent statistical significance compared to cells that were transfected with control siRNA and not treated with 4-ClBQ; # indicates statistical significance compared to cells that were transfected with control siRNA and treated with 4-ClBQ; p < 0.05, n = 3.