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Solvatochromic properties of 2AA and 2CNA-GPP. (A) 2AA-GPP in water (gray line) or 1-propanol (black line) and (B) 2CNA-GPP in water (gray line) or 1-propanol (black line). (C) Difference in fluorescence between matched concentrations of 2CNA-GPP and 2CNA-B(6)PP under typical reaction conditions without enzyme or IPP. Error bars represent one standard deviation from the average value for three samples.
