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2AA-GPP and 2CNA-GPP are effective probes for monitoring UPPS activity. Plate monitoring of reactions of (1, red) UPPSBf, (2, black) UPPSEc, and (3, blue) UPPSVv with (A) 2AA-GPP and (B) 2CNA-GPP. Error bars represent one standard deviation from the average of three samples. Product distributions upon quenching at 3500 s are shown in Figures 2A and 3A.
