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. 2014 Jul 14;53(30):5042–5050. doi: 10.1021/bi500545g

Figure 6.

Figure 6

2AA-GPP utilization is impaired with V. vulnificus UPPS and E. coli UPPS. (A) UPPS concentrations that doubled the 2CNA-GPP UPPSBf rate with the E. coli and V vulnificus proteins were used. (B) Distribution of products form reactions in panel A quenched at 1500 s. (C) Change in fluorescence with 2AA-GPP with all enzyme concentrations used in panel A increased by 3.5-fold. (D) Distribution of products in panel C after quenching at 1500 s: (1, red) UPPSBf, (2, black) UPPSEc, and (3, blue) UPPSVv. Chromatograms 2 and 3 are offset by 2 and 4 min, respectively. Note that the peaks representing flow-through material are cut to give the best view of long chain isoprenoid products.