FIG. 2.

In vitro treatment of P. falciparum cultures with terpenes or FTS and metabolic labeling with [³H]GGPP and [³H]FPP. Parasites were untreated or treated for 48 h with terpenes and FTS and labeled for 18 h with a 3.125-μCi/ml concentration of [³H]GGPP (A to C) or [³H]FPP (D to F) in the presence of the drugs. The parasites were harvested and purified by Percoll gradient, lysed, and analyzed by SDS-PAGE and fluorography. (A and D) Ring forms; (B and E) trophozoite forms; (C and F) schizont forms. Lane 1, untreated parasites; lane 2, 5.5 μM FTS; lane 3, 25 μM farnesol; lane 4, 500 nM nerolidol; lane 5, 0.5 mM limonene; lane 6, 0.1 mM linalool. Molecular size standards are indicated on the left.