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. Author manuscript; available in PMC: 2015 Feb 26.
Published in final edited form as: Dev Genet. 1999;25(4):353–364. doi: 10.1002/(SICI)1520-6408(1999)25:4<353::AID-DVG9>3.0.CO;2-G

Fig. 1.

Fig. 1

Aldh1 and Raldh2 mRNA transcripts stimulate RA synthesis in Xenopus embryos. Xenopus embryos at the 2–4 cell stages were injected with 23 nl of mRNA(0.2 µg/µl) transcribed from either mouse Raldh2 (A), mouse Aldh1 (B), or human ALDH3 (C), or were uninjected as a control (D). Following growth to blastula stage 8, embryos were subjected to an RA bioassay which involves incubating the embryo on a lawn of RA-reporter cells followed by assay for lacZ expression. In each photograph the embryo has been moved aside from its site of incubation, and arrows point to sites of lacZ expression.