Fig 4. Differentiation of LMPCs into fat, bone and smooth muscle cells.
(A) Oil Red O staining of LMPCs isolated from macrocystic cervicofacial (Macro CF) LM and microcystic mesenteric (Micro Mes) LM after 2 weeks in growth media (control) or adipogenic media. (B) Alkaline phosphatase (Alk Phos) staining of LMPCs isolated from macrocystic Macro CF LM and Micro Mes LM after 2 weeks in growth media (control) or osteogenic media. (C) NG2 and alpha smooth muscle actin (αSMA) staining of LMPCs isolated from Micro Mes LM after 2 weeks in growth media (control) or mural cell differentiation (Diff) media. Scale bars: 50μm.