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. 2015 Feb 26;10(2):e0117352. doi: 10.1371/journal.pone.0117352

Fig 5. LMPCs differentiated into abnormal lymphatic endothelial cells.

Fig 5

LMPCs isolated from mixed cervicofacial (Mixed CF) LM, macrocystic mesenteric (Macro Mes) LM and a generalized lymphatic anomaly (GLA) were maintained in growth media (control) or lymphatic endothelial differentiation (LEC Diff) media for two weeks. (A) CD31/VE-cadherin and (B) LYVE1/podoplanin staining. Scale bars: 50μm. (C) VEGFR-1, VEGFR-2, VE-cadherin, Prox1, Podoplanin, LYVE1, and VEGFR-3 qRT-PCR of RNA isolated from LMPCs maintained in growth or LEC differentiation (LEC Diff) media. Data normalized to β-actin qRT-PCR and represented as mean ± s.e.m. * p < 0.05, ** p < 0.005, *** p < 0.0005.