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. 2015 Mar 1;26(5):821–831. doi: 10.1091/mbc.E14-04-0868

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© 2015 Paccez et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Axl-mediated Akt activation is essential for proliferation of OSCC cells. (A) Kyse450 siRNA GFP and WHCO5 siRNA GFP and Axl knockdown cells Kyse 450 siRNA Axl and WHCO5 siRNA Axl were transfected with pcDNA Flag control or pcDNA Flag-Axl plasmids. Total-cell lysates were collected at 48 h posttransfection. The extracts were then analyzed by Western blotting using anti-Axl, anti–phospho-Akt, anti-Akt, anti–phospho-IKKα, anti-IKKα, and anti-GAPDH antibodies after restoration of Axl expression. (B) Reexpression of Axl protein restores proliferation in Kyse450 siRNA Axl and WHCO5 siRNA Axl. Control cells (Kyse450 siRNA GFP and WHCO5 siRNA GFP) and Axl knockdown cells (Kyse 450 siRNA Axl and WHCO5 siRNA Axl) were transfected with pcDNA Flag control or pcDNA Flag-Axl plasmids, and proliferation was evaluated 48-h posttransfection. Data shown are mean ± SD of triplicate independent experiments for each condition; *, p < 0.05.