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. 2015 Mar 1;26(5):832–842. doi: 10.1091/mbc.E14-06-1136

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© 2015 Zappitelli et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — BMP2/4 signaling is increased in Gja1^Jrt/+ in vivo and in vitro. Expression of Bmp2, Bmp4, and Tcf7 was increased in Gja1^Jrt/+ vs. WT (A) trabecular bone at 4 mo of age (n ≥ 8) and in (B) osteogenic stromal cultures at confluence (n ≥ 3). (C) Levels of pSMAD1/5/8 were significantly increased in Gja1^Jrt/+ vs. WT confluent osteogenic stromal cultures. Levels of SMAD1 were unchanged. One representative blot is shown; n ≥ 4. ^#p < 0.1, *p < 0.05, **p < 0.01.