FIGURE 5:

Up-regulated BMP2/4 signaling is responsible for the increased osteoblast marker expression and the increased Pparg2 expression in bone marrow–derived adipocytes and adipogenic precursors in Gja1^Jrt/+ vs. WT mice. (A) The dose of Noggin required for either half (ID₅₀) or maximal knockdown of both Bsp and Ocn expression was higher in Gja1^Jrt/+ vs. WT osteogenic stromal cells. One representative experiment is shown, and samples were run in triplicate; n = 3. (B) Both WT and Gja1^Jrt/+ adipogenic stromal cells grown in the presence of Gja1^Jrt/+ conditioned medium expressed higher levels of Pparg2 vs. those grown with the addition of WT conditioned medium; n ≥ 3. Solid and dashed lines indicate significant differences between cells cultured in either conditioned medium situation in WT and Gja1^Jrt/+ cells, respectively. (C) Expression of Pparg2 declined significantly when cells of either genotype (cultured under adipogenic conditions and with the addition of Gja1^Jrt/+ conditioned medium) were treated with Noggin; n = 5. Asterisks indicate significance between genotypes at that dosage concentration; *p < 0.05, **p < 0.01, and ***p < 0.001. Capital letters indicate significance between WT samples; lowercase letters indicate significance between Gja1^Jrt/+ samples; letters are assigned in alphabetical order according to the dosages (e.g., significant difference vs. dose 0 is denoted A in WT and a in Gja1^Jrt/+). p < 0.05 was considered statistically significant.