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. 2015 Mar 1;26(5):859–873. doi: 10.1091/mbc.E14-06-1086

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© 2015 Chazeau, Garcia, Czöndör, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Formation of actin-rich dendritic filopodia on N-cadherin coated micropatterns. (a, b) Neurons coexpressing actin-GFP and either NcadWT-RFP or NcadΔE-RFP, respectively, were cultured on micropatterned substrates coated with Ncad-Fc and immobilized through Cy5-conjugated anti-Fc antibodies. Images show (from left to right) the Cy5, RFP, GFP, and merge between actin-GFP (green) and micropatterns (red). (c) Enrichment levels of Ncad-RFP and actin-GFP at Ncad-Fc micropatterns in NcadWT-RFP and NcadΔE-RFP conditions. The two conditions were compared by a Mann–Whitney test separately for each staining (actin-GFP or Ncad-RFP). **0.001 < p < 0.01. The number of micropatterns analyzed was 257 for NcadWT-RFP and 291 for NcadΔE-RFP (four cells in each condition). (d) Plot of actin-GFP vs. Ncad-RFP signals for the NcadWT and NcadΔE conditions, respectively. The correlation coefficient was r = 0.83 for NcadWT (p < 0.0001, n = 257 dots) and 0.45 for NcadΔE (p < 0.0001, n = 291 dots).