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. 2015 Mar 1;26(5):901–912. doi: 10.1091/mbc.E14-07-1250

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© 2015 Suraneni et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Actin organization at the leading edge and paxilin localization in wt and ARPC3^−/− fibroblasts. (A) Spreading wt and ARPC3^−/− cells were stained with fluorescent phalloidin and imaged by SIM. The white boxes represent the zoomed regions in the corresponding images. (B) Spreading ARPC3^+/+ (top panels) and ARPC3^−/− cells (bottom panels) were stained with antibodies against paxilin (green), Alexa Fluor 546 phalloidin for F-actin (red) and DAPI to visualize the nucleus (blue). Representative images are shown. Scale bars: (A) wt 2 μm, 1.5 μm (zoom) and ARPC3^−/− 2.5 μm, 1.5 μm (zoom); (B) 20 μm.