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. 2015 Mar 1;26(5):924–937. doi: 10.1091/mbc.E13-12-0721

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© 2015 Eagleson, Pfister, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Removal of animal cap tissue rescues Kif2a MO gastrulation phenotypes. Embryos were injected with water (Control) or Kif2a morpholino (Kif2aMO) at two-cell stage. At stage 8, animal caps of each treatment were removed microsurgically, and the blastopore closure was monitored through low-light time-lapse microscopy (Control-Capless, Kif2aMO-Capless). One embryo was left intact for staging purposes (Control). Failure of blastopore closure in the morphant phenotypes were rescued by removal of the cap, although closure was slightly delayed compared with the control.