FIGURE 5:

Kif2a morphant cellular phenotypes are rescued by Kif2a but not Kif2a(T70A). (A) Confocal micrographs of control, Kif2a-MO, and WT rescue morphants (Rescue Kif2a WT) stained for α-tubulin (green) and DNA (blue). In the control and MO-rescue animal caps, spindles were bipolar, whereas within the Kif2a-MO group, multipolar spindles were abundant. (B) Confocal images of control and Kif2a morphants rescued with a phospho-null Kif RNA (Kif2a T70A) show that Kif2a is mislocalized in the absence of T70 phosphorylation. Animal caps were stained for α-tubulin (green), Kif2a (red), and DNA (blue). (C) Confocal measurements of spindle lengths were calibrated as a pole-to-pole length (note spindle lines). The stage 10.5 Kif2a morphant cells had significantly greater metaphase and anaphase spindle lengths compared with the control and rescue animal cap cells.