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hSNF5-induced NOXA protein expression. A, cells were harvested at the indicated times after infection with Ad-hSNF5 and Ad-GFP. Total cell protein (30 μg) were separated on a 4% to 20% SDS-PAGE and probed with either anti-SNF5, anti-β-actin, or anti-NOXA. un; uninfected control. B, cells were transfected with the designated plasmids and selected in neomycin for 2 weeks. The results represent the colony numbers from 3 independent experiments; bars, ±SD.
