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Molecular diagnosis of Plasmodium infection by nested-PCR in organ fragments of dead monkey. DNA extracted from spleen and liver samples of dead wild Alouatta g. clamitans found near the campus of the CPRJ (sample MP1943) was amplified by using primers specific for P. vivax (Pv). Agarose gel, 1.5% stained with ethidium bromide. C+ − positive control for P. vivax and CN – negative control (without DNA), M – Ladder 100 bp.
