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. 2015 Feb 22;8:27. doi: 10.1186/s13068-015-0203-5

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© Calusinska et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characteristics of the different glucose fermentations for Clostridium butyricum CWBI 1009 performed under unregulated-pH and at fixed pH 7.3 and 5.2. (A, B, C) Growth curve (OD) and pH. (D, E, F) Hydrogen production rate (L/h) and cumulative hydrogen production (L) profiles. (G, H, I) Glucose utilisation and profiles of soluble metabolites (mM). The error bars refer to the three independent replicate experiments. Fermentations were performed in 20-L batch bioreactors with glucose as a substrate (10 g/L for unregulated-pH fermentations and 5 g/L for fixed-pH fermentations).