Table 1.
List of C. butyricum CWBI 1009-specific primers and probes used in this study
| Target gene | Primer/probe | Sequence 5’ → 3’ | Length (bp) | Ref. |
|---|---|---|---|---|
| recA | RecA-ButF | AAGCATTAGTGCGTTCTGGAG | 97 | [41] |
| RecA-ButR | GAATCTCCCATTTCCCCTTC | |||
| hydA2 | ButA2F | ATAGTTGCAATGGCTCCTGC | 250 | This study |
| ButA2R | TTTCTGCTTGCCTAACCCAT | |||
| hydA8 | ButA8F | TCTTTGGAGTTACAGGGGGA | 188 | This study |
| ButA8R | TTCAGCATTTGCAAGACCAC | |||
| hydB2 | ButB2F | TGGTGGTGTATCAACTGCTG | 168 | This study |
| ButB2R | TTGCATCCCATTCCTTCAAT | |||
| hydB3 | ButB3F | CAATGGTTGCTACAGGCAGA | 168 | This study |
| ButB3R | CAAAAGCATCGAATAACGCA | |||
| 16S rRNA | 16SButF | CCTGCCTCATAGAGGGGAAT | 143 | This study |
| 16SButR | GAGCCGTTACCTCACCAACT | |||
| HP16SButa | CCGCATAAGATTGTAGTACCGCATGGTACA | |||
| nifH | NifHButF | CATCAGCATTGGCTGAGATG | 206 | This study |
| NifHButR | TGGTTCTGGTCCTCCTGATT |
aHP: hydrolysis probe.