Table 3. Overview workflow for microarray experiment.
Culture condition | CFU Ratio | Sample time point experiment | Induction | Biological replicates |
---|---|---|---|---|
Pure culture EHEC NIPH-11060424 | NA | mid-logarithmic phase (OD = 0.5) | No | >3 |
Co-culture EHEC NIPH-11060424/B. thetaiotaomicron) | 1/100 | mid-logarithmic phase (OD = 0.5) | No | 3 |
Pure culture EHEC NIPH-11060424 in spent medium from B. thetaiotaomicron | NA | mid-logarithmic phase (OD = 0.5) | No | 3 |
Induced pure culture EHEC NIPH-11060424 | NA | 3 hours after induction | MMC | 3 |
Induced co-culture EHEC NIPH-11060424/B. thetaiotaomicron | 1/100 | 3 hours after induction | MMC | 3 |
Induced culture EHEC NIPH-11060424 in spent medium from B. thetaiotaomicron | NA | 3 hours after induction | MMC | 3 |