Table 4.

Effect of physical activity on the molecules of energy sensing pathway in mammary carcinomas^*

Group	Sedentary Control	Restricted Energy	Physically Active
pAMPK(Thr172)	14.1 ± 1.7	15.5 ± 1.1	17.9 ± 2.6
AMPK	19.0 ± 2.6	17.5 ± 1.3	15.0 ± 1.3
pAMPK/AMPK	0.74 ± 0.03	0.89 ± 0.04	1.19 ± 0.06††
pACC(Ser79)	20.3 ± 1.2	26.8 ± 2.9	26.6 ± 2.2
ACC	22.8 ± 1.4	24.9 ± 4.8	20.8 ± 2.3
pACC/ACC	0.89 ± 0.07	1.08 ± 0.13	1.28 ± 0.09†
pAkt(Ser473)	13.3 ± 0.9	13.9 ± 1.9	9.7 ± 0.8
Akt	43.1 ± 3.1	49.7 ± 6.0	39.8 ± 3.0
pAkt/Akt	0.31 ± 0.02	0.28 ± 0.02	0.24 ± 0.01†

^*The levels of proteins (relative absorbance unit x 10^x4) or the ratio of phosphorylated to non phosphorylated protein are shown; values are mean ± SEM, n=7 per treatment group. The images shown in Figure 4 are those directly acquired from the ChemiDoc work station that is equipped with a CCD camera having a resolution of 1300 x 1030. The normalized intensity data from the ChemiDoc were evaluated; statistical analyses were done on the ranks of the absorbance data via multivariate analysis of variance as described in the Materials and Methods section. pAMPK: phosphorylated AMP-activated protein kinase; pACC: phosphorylated acetyl-CoA-carboxylase. Compared to sedentary control,

^†p<0.05,

^††p<0.01.