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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Extremophiles. 2014 Dec 4;19(2):269–281. doi: 10.1007/s00792-014-0712-3

Figure 5. PF2064 locus organization and gene response during growth phase transitions in WT and Mutant.

Figure 5

(A)The PF2064 locus includes small hypothetical ORFs, bacteriocin synthesis related genes (yellow), radical SAM proteins (dark blue), transporters (light blue), toxins/antitoxins (pink), and a transcriptional regulator containing HTH domain (grey). PF2065 is the last annotated gene in P. furiosusgenome and hence, PF0001-0005 genes are depicted to be a part of this locus. Gene organization was generated using Vector NTI (Invitrogen). (B) The amino acid sequence (46 residues) of a putative small peptide, presumably bacteriocin-like, discovered in the locus, its N and C termini indicated in red. Cysteines are highlighted in black. Amino acids are noted by their one letter code. (C) Transcriptional response of genes in the PF2064 locus. Red indicates higher transcript levels (log2(fold change)>1), green indicates lower (log2(fold change)<-1), and black denotes average transcript levels. The color scale used is the same as in Figure 4.