Table 1.

ORFs differentially transcribed 10-fold or more for P. furiosus wild-type (WT) and mutant growth contrasts

Gene ID	Fold change
	Mutant vs. WT
	10h	14h	24h	Annotation	Comment
PF0121	↓9.2	↓17.1	↓18.4	aspartate aminotransferase	PF0122 mutated (D)
PF0207	2.5	2.6	↓9.8	argininosuccinate synthase	PF0209 mutated (S)
PF0333	↓4.0	↓14.9	↓2.1	flagella-related protein g	Flagella biosynthesis; PF0332, PF0334, PF0337 mutated (FS)
PF0335	↓4.3	↓16.0	↓3.7	flagella-related protein d
PF0336	↓11.3	↓45.3	↓7.0	hypothetical protein
PF0351	↓7.0	↓13.0	↓12.1	CRISPR-related protein	Both mutated (FS), CRISPR-related
PF0352	↓13.9	↓19.7	↓24.3	RNA-binding protein (RAMP)
PF1056		2.6	↓13.9	Asp-semialdehyde dehydrogenase	Asp metabolism
PF1133	5.7	8.6	13.0	hypothetical protein (68 aa)	PF1132 mutated
PF1266	↓10.6	↓2.5	↓13.0	cystathionine gamma-synthase	Met biosynthesis
PF1267	↓12.1	↓3.5	↓13.0	hypothetical protein	Mutated
PF1268	↓24.3	↓3.5	↓24.3	homocysteinemethyltransferase	Mutated
PF1269	↓13.0	↓2.0	↓7.5	methionine synthase	Met biosynthesis
PF1472		2.5	↓9.8	aspartate/serine transaminase	PF1474 mutated
PF1480		3.0	↓11.3	formaldehyde:ferredoxinoxidoreductase wor5	Mutated
PF1528			14.9	GInamidotransferase, PdxT
PF1529		2.3	13.0	pyridoxine biosynthesis protein
PF1549	↓10.6	↓11.3	↓14.9	RNA 3'-terminal-phosphate cyclase	PF1553 mutated
PF1664		2.8	↓13.9	phosphoribosyl-AMP cyclohydrolase
PF1678		3.0	↓11.3	2-isopropylmalate synthase	Lys, Leu biosynthesis
PF1679		2.8	↓14.9	3-isopropylmalate dehydratase
PF1680		2.6	↓13.9	3-isopropylmalate dehydratase
PF1682		2.1	↓10.6	ribosomal protein s6 modification protein
PF1683		2.5	↓9.8	N-acetyl-γ-glutamyl-P-reductase
PF1684		2.1	↓10.6	acetylglutamate kinase
PF1713	2.3		↓10.6	carbamoyl phosphate synthase	Pyrimidine metabolism
PF2064		↓13.0	↓26.0	arylsulfatase regulatory protein	SAM radical family

D = deletion; S = in-frame substitution; FS = Frame shift