Table 1.

Effects of glucocorticoid receptor targeting on HPA axis activity, behavior, and metabolic phenotypes

Phenotype	GRNesCre	FBGRKO Male	FBGRKO Female	GRCaMKCre	Sim1Cre- GRe3Δ	Sim1Cre- GRe2Δ	GRflox+/+; Sim1Cre+	GRflox+/−; Sim1Cre+	GRPOMCCre
GR floxed	Exon 3	Exon 1C-2	Exon 1C-2	Exon 3	Exon 3	Exon 1C-2	Exon 2	Exon 2	Exon 3
Primary region	CNS	Forebrain	Forebrain	CNS + PIT	PVN	PVN	PVN/Lung	PVN	PIT
GR loss		75–100%	75–100%		87%	43%		70%	90%
Lethality	None	None	None	Postnatal	None	None	Embryonic	None	None
Postnatal Day 6
PVN CRH	↑			↑	↑				↓
PVN AVP									↑
POMC mRNA				↑					↑ N
Plasma ACTH				↑N					↑ N
Plasma CORT	↑			↑					↑ N
Weight	↓			↓	↓pw				↓
Skin pigm.	⬄			↓
Skin fat	⬄			↓sc
Thymus weight	⬄			↓					⬄
Spleen weight	⬄			↓
Adult
PVN CRH	↑	⬄B, ↑S	⬄		↑	⬄		↑	⬄
PVN AVP	⬄	↑	⬄
Plasma ACTH	↑PIT, ➘N	↑P/ S	⬄		↑N/P, ⬄S	⬄ N/P/S		↑B	⬄
Plasma CORT	↑N/P	➚N, ↑P/S	⬄		↑N/P/S	⬄ N/P/S		↑B	⬄
		↑N, ⬄P
DST		Impaired			Impaired	⬄			Impaired
Weight	↓	⬄			↑	⬄		↓	⬄
Fat Mass	↓				↑	⬄
Anxiety	↓	↓			⬄	⬄			↑
Despair	↓	↑	⬄		⬄	⬄			↓
Stress-coping	↑	↓	⬄		⬄	⬄			↑
Locomotor activity	⬄	↑EZM			↓OF	⬄			⬄
References	(Tronche et al., 1999) (Kellendonk et al., 2002) (Erdmann et al., 2008)	(Boyle et al., 2005) (Boyle et al., 2006) (Furay et al., 2008)	(Solomon et al., 2012)	(Erdmann et al., 2008)	(Laryea et al., 2013)		(Jeanneteau et al., 2012)		(Schmidt et al., 2009) (Wagner et al., 2011)

PIT, pituitary; Prot, protein; N, nadir; P, peak; S, stress; B, Basal; sc, subcutaneous; pw, pre-weaning; EZM, elevated zero maze; OF, open field; ↑, increase; decrease; ➚, trend towards increase; ➘, trend towards decrease; ⬄, similar to controls; DST, dexamethasone suppression test.