Table 2.
Features of protein and rRNA genes in parental (Nicotiana tabacum (Nt) and Hyoscyamus niger (Hn)) and cybrid (Cyb) mitochondrial genomes
| Gene namea | Length (bp)b | Hn/Nt differencesc | Hn/Nt differences in coding regions |
Hn/Nt differences in predicted editing sites | # intact genes |
Origin of cybrid genes | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Synonymous | Non-synonymous | Hn | Nt | Cyb | ||||||
| atp1 | 1530 | 25 | 19 | 6 | 0 | 1 | 1 | 2 | chimeric & Nt | |
| atp4 | 597 | 5 | 1 | 4 | 0 | 1 | 1 | 1 | Nt | |
| atp6 | 711 | 3 | 2 | 1 | 0 | 1 | 1 | 1 | Nt | |
| atp8 | 471 | 4 | 2 | 2 | 0 | 1 | 1 | 1 | Nt | |
| atp9 | 234 | 2 | 0 | 2 | 0 | 1 | 1 | 2 | Nt & Hn | |
| ccmB | 621 | 2 | 1 | 1 | 0 | 1 | 1 | 2 | Nt | |
| ccmC | 753 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | Hn | |
| ccmFc | 2271 | 14 | 2 | 0 | 0 | 1 | 1 | 1 | Nt | |
| ccmFn | 1740 | 37 | 7 | 30 | 2 | 1 | 1 | 2 | Hn | |
| cob | 1182 | 3 | 2 | 1 | 0 | 1 | 1 | 1 | Nt | |
| cox1 | 2551 | 11 | 9 | 2 | 2 | 1 | 1 | 2 | Hn | |
| cox2 | 2143 | 8 | 2 | 4 | 1 | 1 | 1 | 1 | chimeric | |
| cox3 | 798 | 2 | 1 | 1 | 0 | 1 | 1 | 1 | Nt | |
| matR | 1977 | 5 | 2 | 3 | 0 | 1 | 1 | 1 | chimeric | |
| mttB | 840 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | Nt | |
| nad1d | x1 | 387 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | Nt |
| x2-3 | 1485 | 12 | 1 | 0 | 0 | 1 | 1 | 1 | Nt | |
| x4 | 59 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | Hn | |
| x5 | 259 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | Nt | |
| nad2 | x1-2 | 1575 | 6 | 0 | 0 | 0 | 1 | 2 | 2 | Nt |
| x3-5 | 4796 | 24 | 1 | 0 | 0 | 1 | 1 | 1 | Nt | |
| nad3 | 357 | 4 | 2 | 2 | 0 | 1 | 2 | 2 | Nt | |
| nad4 | 8512 | 28 | 1 | 0 | 0 | 1 | 1 | 1 | chimeric | |
| nad4L | 303 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | Nt | |
| nad5 | x1-2 | 2290 | 8 | 1 | 1 | 0 | 1 | 1 | 1 | Nt |
| x3 | 22 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | Nt | |
| x4-5 | 1633 | 20 | 1 | 0 | 0 | 2 | 1 | 1 | chimeric | |
| nad6 | 687 | 3 | 2 | 1 | 0 | 1 | 1 | 1 | Nt | |
| nad7 | 5294 | 37 | 0 | 0 | 0 | 1 | 1 | 1 | chimeric | |
| nad9 | 573 | 2 | 0 | 2 | 0 | 2 | 1 | 1 | Nt | |
| rpl2 | 2898 | 36 | 4 | 4 | 0 | 1 | 1 | 1 | Nt | |
| rpl5 | 555 | 9 | 4 | 5 | 1 | 1 | 1 | 1 | Nt | |
| rpl10 | 480 | 4 | 2 | 2 | 0 | 1 | 1 | 2 | Hn | |
| rpl16 | 516 | 3 | 1 | 2 | 0 | 1 | 1 | 1 | Hn | |
| rps1 | 651 | 3 | 2 | 1 | 0 | 1 | 1 | 1 | Nt | |
| rps3 | 3469 | 27 | 4 | 6 | 0 | 1 | 1 | 1 | Hn | |
| rps4 | 1050 | 6 | 5 | 1 | 0 | 1 | 1 | 1 | Nt | |
| rps10 | 1138 | 4 | 1 | 2 | 0 | 1 | 1 | 2 | Hn | |
| rps12 | 378 | 2 | 1 | 1 | 0 | 1 | 1 | 1 | Nt | |
| rps13 | 351 | 3 | 1 | 2 | 0 | 1 | 1 | 1 | Nt | |
| rps19 | 285 | 3 | 1 | 2 | 0 | 1 | 1 | 2 | Hn | |
| rrn5 | 119 | 1 | NA | NA | ND | 1 | 1 | 1 | Nt | |
| rrn18 | 1902 | 4 | NA | NA | ND | 1 | 1 | 1 | Nt | |
| rrn26 | 3432 | 15 | NA | NA | ND | 2 | 2 | 1 | chimeric | |
| sdh3 | 327 | 3 | 1 | 2 | 0 | 1 | 3 | 3 | Nt | |
| sdh4 | 378 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | Nt | |
a
Intron-containing genes are in bold face.
b
Gene lengths are from the cybrid mtDNA.
c
These are nucleotide differences, excluding gaps.
d
nad1 is chimeric in the sense that the four separately-transcribed portions of this trans-spliced gene are derived from different parents.
NA, not available; ND, not determined. [Author, please check inserted text ‘NA, not available; ND, not determined.’ is correct. (or should it read ‘not applicable’?]