Table 3.
Recombination events between parental mitochondrial genomes (Nicotiana tabacum (Nt) and Hyoscyamus niger (Hn)) in the cybrid Nt(+Hn)
| Event # | Contig # | Recombining regions | Crossover (CO) or gene conversion (GC) event | Location of recombination | SBP test supporta | Length of gene conversion tract | # of recombination productsb | ||
|---|---|---|---|---|---|---|---|---|---|
| # bp | Identity | Genic | Breakpointa | ||||||
| 1b | 1 | 536 | 99% | CO | no | 417-587 | NS | 2b | |
| 2 | 1 | 1824 | 95% | CO | no | 16,493 | 100% | 1 | |
| 3 | 1 | 909 | 99% | CO | no | 41,000-41,150 | NS | 1 | |
| 4 | 1 | 398 | 99% | CO | atp1 | 55,160-55,330 | NS | 1 | |
| 5 | 1 | 8987 | 98% | GCc | no | 90,730-91,400 | NA | 18-371 | 1 |
| 6 | 1 | 4107 | 96% | CO | no | 117,050 | 100% | 1 | |
| 7 | 1 | 1597 | 98% | CO | no | 131,635 | 100% | 1 | |
| 8 | 1 | 226 | 99% | CO | no | 144,226 | 96% | 1 | |
| 9 | 1 | 862 | 98% | CO | no | 144,100-144,350 | NS | 1 | |
| 10 | 1 | 3815 | 99% | CO | no | 184,360 | 100% | 1 | |
| 11 | 1 | 199 | 99% | CO | no | 207,461 | 86% | 1 | |
| 12 | 1 | 3385 | 97% | GCc | no | 215,950-219,100 | NA | 2866-3150 | 1 |
| 13 | 1 | 7042 | 97% | CO | nad4 | 245,122 | 100% | 1 | |
| 14 | 1 | 7042 | 97% | GCc | no | 248,650-249,088 | NA | 151-344 | 1 |
| 15b | 2 | 634 | 99% | CO | trnQ | 8,592-8,794 | NS | 2b | |
| 16 | 2 | 1721 | 97% | CO | no | 9,245 | 100% | 1 | |
| 17b | 2 | 1691 | 98% | CO | no | 17,420 | 100% | 2b | |
| 18 | 2 | 678 | 99% | CO | no | 18,357-18,600 | NS | 1 | |
| 19 | 2 | 5716 | 99% | GCc | nad7 | 48,068-49,444 | NA | 168-1376 | 1 |
| 20 | 2 | 1669 | 96% | CO | no | 60,844 | 100% | 1 | |
| 21 | 2 | 1852 | 98% | CO | no | 72,300 | 100% | 1 | |
| 22 | 2 | 103 | 100% | CO | no | 89,332-89,435 | NS | 1 | |
| 23 | 2 | 2183 | 94% | CO | no | 114,942 | 100% | 1 | |
| 24 | 2 | 1003 | 99% | CO | no | 179,018-179,289 | NS | 1 | |
| 25 | 2 | 3385 | 99% | CO | no | 190,346 | 100% | 1 | |
| 26 | 2 | 199 | 99% | CO | no | 198,710-198,813 | NS | 1 | |
| 15b | 2 | 634 | 99% | CO | no | 216,921-216,928 | NS | 2b | |
| 27 | 2 | 1524 | 99% | GCc | no | 220,198-221,579 | NA | 1020-1381 | 1 |
| 28 | 3 | 3488 | 99% | CO | cox2 | 12,725 | 100% | 1 | |
| 29 | 3 | 1861 | 99% | CO | nad5 | 32,629 | 100% | 1 | |
| 17b | 3 | 1691 | 98% | CO | no | 42,718 | 100% | 2b | |
| 1b | 3 | 536 | 99% | CO | no | 48,170-48,240 | NS | 2b | |
| 30 | 4 | 9000 | 99% | CO | no | 22,140 | 100% | 1 | |
| 31 | 4 | 100 | 99% | CO | no | 35,525-35,624 | NS | 1 | |
| 32 | 5 | 3563 | 99% | CO | rrnL | 2,021 | 100% | 1 | |
| 33 | 5 | 6374 | 99% | CO | matR | 18,482 | 100% | 1 | |
| 34 | 6 | 2866 | 99% | CO | no | 4,726 | 95% | 1 | |
| 35 | 6 | 921 | 99% | CO | no | 14,413 | 99% | 1 | |
Breakpoint locations were calculated by the SBP (Single BreakPoint Recombination) program when they were significant as determined by this program. In those cases in which the SBP test was not significant (NS) but a crossover was nonetheless apparent because parental-unique sequences were found at each side of the putative crossover, the region of the breakpoint was estimated based on the length of the cybrid region that is identical to both parents and which is flanked by regions containing shared polymorphisms with one parent on one end and the other parent on the other end.
For only three of the 35 recombination events were two recombination products detected. These events (#'s 1, 15, and 17) therefore appear twice in this table.
The GeneConv program was significant (P<0.01) for these gene conversion events.
NA, not available. [Author, please check inserted text ‘NA, not available’ is correct (or should it read ‘not applicable’?]