Fig. 4. Acyl-CoA synthetase (ACSL1) overexpression increases neutral lipid accumulation and relieves ER stress.
(A) qPCR and Western Blot data demonstrating successful adenovirus-mediated overexpression of ACSL1 in AC16 cells. (B) Representative images of Oil-red-O stained AdGFP or AdACSL1-infected cells treated with either palmitate (PA) or oleate (OA) (magnification x10). (C–E) ACSL1-mediated reduction in palmitate-induced expression of ER stress markers ATF6 (C), CHOP (D) and GRP78 (E). N=3–4 per condition. Data are presented as mean±SEM. *P<0.05 BSA-control versus palmitate within groups, #P<0.05 ACSL1+PA versus GFP+PA, n=4.