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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Dec;1841(12):1648–1655. doi: 10.1016/j.bbalip.2014.09.012

Fig. 5. ATGL-mediated reduction in triglyceride (TAG) accumulation induces ER stress.

Fig. 5

(A) qPCR data demonstrating successful adenovirus-mediated overexpression of ATGL in AC16 cells. Mean±SEM, n=7–8, *P<0.05. MOI: 10. (B) Oil-red-O staining of AdGFP or AdATGL-infected AC16 cells in the absence or presence of oleate (magnification x10). (C–D) ATGL overexpression reduces intracardiomyocellular TAG levels and increases FFA levels. Data are presented as fold change compared to the GFP control group. n=6, error bars represent SEM. *P<0.05 BSA-control versus palmitate within groups, #P<0.05 ATGL versus GFP. TAG: triacylglycerol, NEFA: non-esterified FA. (E–H) ATGL-mediated increase in ER stress markers ATF6 (E), CHOP (F), GRP78 (G) and GRP94 (H) in AC16 cells treated with oleate. *P<0.05 BSA-control versus palmitate within groups, #P<0.05 ATGL versus GFP, n=6, error bars represent SEM.

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