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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Brain Behav Immun. 2014 Dec 16;45:98–108. doi: 10.1016/j.bbi.2014.12.015

Figure 5. CXCR4+CD45− BMMNCs express higher levels of pluripotency marker mRNA than do unfractionated BMMNCs in vitro.

Figure 5

(A) Representative gel image of RT-PCR shows the gene expression of Nanog and Oct4 in unfractionated BMMNCs, CXCR4+CD45− BMMNCs, and CXCR4+CD45+ BMMNCs. The housekeeping gene GAPDH served as an internal control. The expression levels of Nanog and Oct4 were too low to detect in CXCR4+CD45+ BMMNCs. (B and C) Quantification of gene expression levels of Nanog (B) and Oct4 (C) in the different cell populations. n=6/group; *p<0.05, ***p<0.001 vs. unfractionated BMMNCs; one-way ANOVA followed by Tamhane’s T2 test.