Figure 6.

The effects of Lsd1 conditional inactivation on erythropoiesis and chromatin occupancy by TR2 and TR4 and corepressors. (A) Lsd1^f/f Lin⁻ BM cells were mock-infected (Cre⁻) or infected with Ad-Cre (Cre⁺) on days 0 and 2 of culture. An aliquot of cells was stained daily with anti-CD71 and anti-Ter119 antibodies prior to flow cytometric analyses. Conditional loss of Lsd1 in Lin⁻ BM cells, which had been exposed to Ad-Cre infection, led to far fewer mature CD71⁻Ter119⁺ cells and an increased number of immature CD71⁺Ter119⁺ cells. (B-E) LSD1 inactivation obliterated its chromatin occupancy, as expected, and of its partner CoREST. The binding of TR2/TR4 and other corepressor components to the β-type globin gene promoters were either unchanged or quite modestly reduced in infected Lsd1^f/f Lin⁻ cells.