FIG. 5.

Gel mobility shift assays of purified Nrd1 and Nab3 proteins. Details of the protein purification, probe sequences, and binding reaction conditions are described in Materials and Methods. Each reaction mixture contained a 2 nM concentration of the labeled RNA probe and, from left to right in each set, 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5.0, or 10 μM purified protein.