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Cofactor Binding
(A) Differential scanning fluorimetry (thermal shift) analysis of HYPE E234G (left) and WT (right) in the absence (control) and presence of indicated compounds. The data are representative for two independent experiments with the same relative difference in Tm.
(B) Binding of ADP to the E234G (E234G, residues 103–445) (left) and WT variant (WT, residues 103–445) (right) was measured by ITC. The indicated errors represent the error of the fit.
