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. 2004 Jul;24(14):6215–6230. doi: 10.1128/MCB.24.14.6215-6230.2004

FIG. 3.

FIG. 3.

Genetic interactions of histone H2A-AQE mutations with mutations in DNA repair genes. (A to D) Survival of cells carrying various combinations of mutations in H2A (hta1-S129A and hta2-S128A, indicated as hta1 hta2) and DNA repair (rad22Δ, rhp51Δ, rad32Δ, rqh1Δ, pku70Δ, and lig4Δ) genes after exposure to the indicated doses of IR. For data in panels C and D, G1 indicates that cells were arrested in G1 by nitrogen starvation for 48 h prior to IR treatment (see Materials and Methods), while G2 indicates exponentially growing cells. Strains used were rad22Δ (TMN3317), rad22Δ hta1 hta2 (TMN3318), rhp51Δ (TMN3319), rhp51Δ hta1 hta2 (TMN3320), rqh1Δ (TMN3301), rqh1Δ hta1 hta2 (TMN3302), rad32Δ (TMN2800), rad32Δ hta1 hta2 (TMN3321), wild type (TMN2665), hta1 hta2 (TMN3293), pku70Δ (TMN3001), pku70Δ hta1 hta2 (TMN3322), lig4Δ (PS2818), lig4Δ hta1 hta2 (TMN3323), rhp51Δ pku70Δ (TMN3419), and rhp51Δ pku70Δ hta1 hta2 (TMN3420). Error bars indicate standard deviations from at least three experiments. (E) A plasmid repair assay to measure NHEJ efficiency was carried out for the indicated strains. Relative colony numbers obtained from transformation with cut versus uncut plasmid are plotted. Strains used were wild type (TMN2663), hta1 hta2 (TMN3292), pku70Δ (TMN3001), pku70Δ hta1 hta2 (TMN3322), lig4Δ (PS2818), and lig4Δ hta1 hta2 (TMN3323). Error bars indicate standard deviations from at least three experiments. (F) Generation times for indicated strains, determined from two independent experiments. Error bars indicate differences between two experiments. Strains used were the same as those used for panels A and B, except for wild-type (TMN3296), hta1 hta2 (TMN3291), rad22Δ (TMN3460), and rad32Δ (TMN3461) strains.