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. 2015 Jan 13;290(9):5256–5266. doi: 10.1074/jbc.M114.618496

FIGURE 1.

FIGURE 1.

JLP deficiency has no effect on splenic lymphocyte numbers and B cell phenotypes. A, jlp−/− and jlp+/+ mice had similar spleen volumes. Spleens were obtained from 8-week-old mice, and one representative result is shown. B, analysis of absolute numbers of splenic B lymphocytes (CD19+) and T cells (CD3+) from four pairs of age- and weight-matched jlp+/+ and jlp−/− mice. Horizontal bars are mean values. C, expression of JLP in splenic B lymphocytes from jlp+/+ and jlp−/− mice. Purified splenic B lymphocyte lysates were subjected to SDS-PAGE and analyzed by Western blotting. HeLa cell extract (Hela CE) and actin were used as positive and loading controls, respectively. D, phenotype analysis of splenic B lymphocytes from two mouse genotypes with or without LPS (1 μg/ml) stimulation for 24 h. B lymphocytes were stained with antibodies specific to CD19, CD40, CD80, CD86, and MHC-II. Cells were counted with a flow cytometer, and the histograms represent surface expression of the indicated markers on gated CD19+ B lymphocytes from jlp+/+ (solid black lines) and jlp−/− (dashed gray lines) mice. The filled curves are negative controls. The results from statistical analysis of the mean fluorescence index (MFI) of each marker from three independent experiments are shown. Error bars indicate S.D.