TABLE 3.
Summary of Rad16 and Rad7 mutant data
| Allele | UV sensitivitya in background:
|
Coimmunoprecipitationb in background:
|
Location of mutation | ||
|---|---|---|---|---|---|
| RAD23+ | rad23Δ | RAD23+ | rad23Δ | ||
| Rad16 | |||||
| K216A | Intermediate | Intermediate | + | ATPase (Walker A) | |
| C537A | Same as rad16Δ | Same as rad16Δ rad23Δ | − | RING domain | |
| C540A | Intermediate | Same as rad16Δ rad23Δ | − | RING domain | |
| C557A | Wild type | Same as rad16Δ rad23Δ | − | RING domain | |
| C560A | Intermediate | Same as rad16Δ rad23Δ | − | − | RING domain |
| Y564A | Wild type | Same as rad16Δ rad23Δ | + | + | RING domain |
| C580A | Intermediate | Same as rad16Δ rad23Δ | − | RING domain | |
| C540A,K216A | Same as rad16Δ | Same as rad16Δ rad23Δ | − | RING and Walker A | |
| C540A,C580A | Same as rad16Δ | Same as rad16Δ rad23Δ | − | RING domain | |
| Y564A,K216A | Intermediate | Same as rad16Δ rad23Δ | + | + | RING and Walker A |
| Rad7 | |||||
| I37A | Wild type | Intermediate | + | + | F-box |
| W41A | Wild type | Same as rad23Δ | F-box | ||
| K40A,Q43A | Wild type | Intermediate | + | + | F-box |
| Δ37-43 | Wild type | Same as rad7Δ rad23Δ | + | + | F-box deletion |
a
Intermediate, the strain is UV sensitive compared to the wild-type strain but not as UV sensitive as a strain with a deletion of the gene of interest; same as rad16Δ, the UV sensitivity of the strain carrying this allele is the same as the UV sensitivity of a strain with a deletion of the gene.
b
+, Rad16 and Rad7 coimmunoprecipitate; −, the two proteins do not coassociate. If no result is shown, the combination was not tested.